![]() A perchlorate reduction pathway would enable S. cerevisiae through recombinational cloning. Using genes obtained from perchlorate reducing bacteria Azospira oryzae and Dechloromonas aromatica, we plan to assemble this pathway directly within S. We designed a perchlorate reduction pathway, which sequentially reduces perchlorate to chloride (Cl−) and oxygen (O2), for implementation in the yeast Saccharomyces cerevisiae. Devising methods to lessen perchlorate contamination is crucial to minimizing the health risks associated with human exploration and colonization of Mars. Even in trace amounts, perchlorates are toxic to humans and have been implicated in thyroid dysfunction. Perchlorate Reduction by Yeast for Mars Exploration Martian soil contains high levels (0.6 percentage by mass) of calcium perchlorate (Ca(ClO4)2), which readily dissociates into calcium and the perchlorate ion (ClO4−) in water. ![]()
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